Macherey-Nagel™ NucleoSpin™ RNA Blood Midi

Midi spin kit for superior isolation of total RNA from fresh or frozen whole blood (human or animal) from common blood collection tubes. Direct blood lysis process does not require the use of a selective erythrocyte lysis.

Brand:  Macherey-Nagel™ 740210.20

Product Code. 15128584

Description

NucleoSpin™ RNA Blood Midi kit achieves superior RNA yield and quality in a format that provides convenient handling and flexible sample volume and throughput.

• Direct total blood lysis—Very simple and convenient procedure
• No selective erythrocyte lysis at 4 °C—Complete processing at room temperature
• Superior RNA yield and quality from up to 1.3 mL whole blood
• Efficient on-column DNA removal for an increased sensitivity in downstream applications
• Compatible with common blood collection tubes and anticoagulants (e.g., EDTA, citrate, and heparin)

Specifications

• Format: Midi Spin Column
• Elution Volume: 200 - 400 μL
• Efficiency: Binding capacity: 700 μg
• For Use With (Equipment): Vortex Mixer, Centrifuge
• Includes: NucleoSpin RNA Blood Midi Columns with Collection Tubes, Collection Tubes (15 mL), buffers, RNase-free rDNase, Liquid Proteinase K
• Purity: A₂₆₀/A₂₈₀: 1.9 - 2.1
• Test Time: 75 min/6 preps

• Isolation Technology: Silica Membrane
• Sample Type: Whole Blood (Fresh or Frozen)
• Final Product Type: Total RNA
• For Use With (Application): RNA isolation
• No. of Reactions: 20 Preps
• Sample Size: 400 - 1300 μL
• Yield: 4 - 26 μL

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• Respiratory sensitiser Category 1
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Supplemental information

• MIXTURE LIST-Contains : guanidinium thiocyanate; ethanol (diluted < 20 %) (denaturedwith 1%IPA/1%MEK, acc.2016/1867/EU); pr