Macherey-Nagel™ Nucleospin™ Plasmid Transfection-grade

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Complete kit includes mini spin columns for an ultra-fast workflow.

Product Code	Brand	No. of Samples
15503636	Macherey-Nagel™ 740490.50	50
15593626	Macherey-Nagel™ 740490.250	250

Description

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

• Purifies high-copy plasmids for transfection
• Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
• Rapid purification from E. coli via manual, vacuum or centrifugation processing
• Ultra-fast procedure using mini spin columns simplifies the work-flow
• Downstream applications: transfection of common cell lines (lipofection, electroporation)
• Also suitable for standard molecular biology application, such as cloning and sequencing

Specifications

• Bacterial Endotoxins: <50 EU/ng DNA
• Format: Mini Spin Column
• Sample Type: Bacterial Culture (E. coli, High-copy Plasmids)
• Elution Volume: Elution Volume: 30 - 50 μL
• For Use With (Equipment): Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
• Includes: NucleoSpin Plasmid Transfection-grade Columns, Collection Tubes, Buffers, RNase A
• Sample Volume: Up to 5 mL
• Test Time: 14 min/6 preps

• Content And Storage: Room temperature (18 - 25 °C)
• Isolation Technology: Silica Membrane and Endotoxin-reduction Technology
• Efficiency: Binding capacity: 35 μg
• Final Product Type: Transfection-grade plasmid DNA
• For Use With (Application): Rapid purification of plasmids for transfection
• Purity: A₂₆₀/A₂₈₀: 1.80 - 1.85
• Size: < 15 kbp
• Yield: 15 - 30 μg