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Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit

Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications.

£60.90 - £646.00

Specifications

Color pH Indicator:
Yellow = correct conditions
Green = pH slightly high (adjustment recommended)
Blue = pH too high (adjustment necessary)
Format Mini Spin Column
Isolation Technology Silica Membrane Technology
Efficiency Typical recovery: 70–95%
Quantity 1 Kit
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Products 3
Product Code Brand No. of Reactions For Use With (Equipment) Price Quantity & Availability  
Product Code Brand No. of Reactions For Use With (Equipment) Price Quantity & Availability  
11992462
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Macherey-Nagel™
740609.10
10 Preps TAE or TBE agarose gels, PCR
£60.90
10 reactions
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Estimated Shipment: 23-04-2024
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15116456
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Macherey-Nagel™
740609.240C
240 Preps QIAcube™ compatible
TAE or TBE agarose gels, PCR
£646.00
240 preps
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Estimated Shipment: 23-04-2024
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12303368
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Macherey-Nagel™
740609.50
50 Preps TAE or TBE agarose gels, PCR
£242.00
50 reactions
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Description

Description

NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.

  • Two applications in one kit—One buffer to process both sample types, with only one loading step
  • High recovery for fragments down to 50 bp—Average recovery from 60–90%
  • Minimized elution volume of 15μL—Highly concentrated DNA
  • Binding buffer with pH indicator—Ensures optimal binding conditions
  • Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
  • Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
  • Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
  • Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)

Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer

Specifications

Specifications

pH Indicator:
Yellow = correct conditions
Green = pH slightly high (adjustment recommended)
Blue = pH too high (adjustment necessary)
Silica Membrane Technology
1 Kit
<400μL (PCR reaction mixture)
<400mg (TAE / TBE agarose gel)
10 min/6 preps
Mini Spin Column
Typical recovery: 70–95%
A260/A280: 1.8–1.9
50 bp to ∼20 kbp
Up to ∼15μg DNA
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