Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up

Artikelnummer.	No. of Reactions	Packungsgröße
12303368	50 Preps	50 reactions
11992462	10 Preps	10 reactions
15116456	240 Preps	240 preps

Artikelnummer. 12303368 Lieferant Macherey-Nagel™ Lieferanten-Nr. 740609.50

Beschreibung

Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications.

NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.

• Two applications in one kit—One buffer to process both sample types, with only one loading step
• High recovery for fragments down to 50 bp—Average recovery from 60–90%
• Minimized elution volume of 15μL—Highly concentrated DNA
• Binding buffer with pH indicator—Ensures optimal binding conditions
• Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
• Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
• Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
• Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)

Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer

Spezifikation

• Color: pH Indicator:
  Yellow = correct conditions
  Green = pH slightly high (adjustment recommended)
  Blue = pH too high (adjustment necessary)
• Format: Mini Spin Column
• Isolation Technology: Silica Membrane Technology
• Efficiency: Typical recovery: 70–95%
• Elution Volume: Elution volume: 15–30μL
• For Use With (Equipment): TAE or TBE agarose gels, PCR
• Includes: Binding Buffer NTI (40mL), Wash Buffer NT3 Concentrate (25mL), Elution Buffer NE (13mL), NucleoSpin Gel and PCR Clean-up Columns (50), 2mL Collection Tubes (50)
• No. of Reactions: 50 Preps
• Quantity: 1 Kit
• Purity: A₂₆₀/A₂₈₀: 1.8–1.9
• Sample Volume: <200 μL PCR reaction mixture, <200 mg agarose gel
• Size: 50 bp to ∼20 kbp
• Test Time: 10 min/6 preps
• Yield: Up to ∼15μg DNA

Sicherheit und Handhabung

Produktidentifikator

• NucleoSpin Gel and PCR Clean-up

Signalwort

• Warning

Gefahrenkategorie

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3

Gefahrenhinweis

• H302-Harmful if swallowed.
• H412-Harmful to aquatic life with long lasting effects.

Ergänzende Informationen

• MIXTURE LIST-Contain: guanidinium thiocyanate