Macherey-Nagel™ NucleoSpin™ DNA Stool, Mini kit for DNA from stool

Product Code.	No. of Reactions	unitSize
15573626	50	50 reactions
15563626	10	10 reactions
15859097	250	250 reactions

Product Code. 15573626 Supplier Macherey-Nagel™ Supplier No. 740472.50

Description

Combines enzymatic lysis with mechanical disruption of cell walls for efficient isolation of high-quality genomic DNA from any stool sample, fresh or frozen. Complete kit includes NucleoSpin™ Bead Tubes.

Isolation of genomic DNA from bacterial and epithelial cells from stool samples

• Efficient procedure combining enzymatic lysis and bead disruption
• Isolation of high-quality genomic DNA (microbial and host) from fresh and frozen samples
• Suitable for any stool sample – carnivores, omnivores, and herbivore
• Bead Tubes included for efficient lysis – compatible with most common disruption devices
• NucleoSpin™ Inhibitor Removal Columns ensure highest purity of the isolated DNA
• Downstream applications: PCR, real-time PCR, Southern blotting, microarray technology

Specifications

• Content And Storage: Room temperature (18 - 25 °C)
• Format: Mini Spin Column
• Isolation Technology: Silica MembraneTechnology and Mechanical (Bead Tube) Disruption
• Sample Type: Stool Samples (Herbivores, Omnivores, or Carnivores, Fresh or Frozen)
• Efficiency: Binding capacity: 50 μg
• Elution Volume: Elution volume: 30 - 100 μL
• For Use With (Equipment): Disruption device (e.g. Vortex-Genie 2 with MN Bead Tube Holder), Centrifuge, Vortex mixer
• For Use With (Application): Isolation of genomic DNA from stool samples
• Includes: DNA Stool Columns, Bead Tubes Type A, Inhibitor Removal Columns, Collection Tubes (2 mL), Collection Tubes (2 mL, lid), Buffers
• No. of Reactions: 50
• Purity: A₂₆₀/A₂₈₀: 1.7 - 1.9
• Sample Volume: Approx. 200 mg (180 - 220 mg)
• Size: Fragment size: 200 bp - approx. 50 kbp
• Test Time: 60 min/10 prep
• Yield: Varies by sample type, quality and water content

Safety and Handling

Product Identifier

• NucleoSpin DNA Stool

Signal Word

• Warning

Hazard Category

• Flammable liquid Category 3
• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• Specific target organ toxicity Category 3
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H319-Causes serious eye irritation.
• H336-May cause drowsiness or dizziness.
• H412-Harmful to aquatic life with long lasting effects.
• EUH032-Contact with acids liberates very toxic gas.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash with water thoroughly after handling.
• P273-Avoid release to the environment.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P330-Rinse mouth.
• P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.