Macherey-Nagel™ NucleoSpin™ DNA Insect, Mini kit for DNA from insects

Product Code. 15523626

Brand: Macherey-Nagel™ 740470.10

Description

Combines enzymatic lysis and mechanical disruption of cell walls for efficient isolation of genomic DNA from a wide variety of insects and crustacean samples (fresh, frozen, dried or ethanol preserved). Complete kit includes NucleoSpin™ Bead Tubes.

Isolation of genomic DNA from insects and crustaceans

• Fast and convenient procedure combining enzymatic lysis and bead disruption
• Suitable for any insect or crustacean (e.g., fruit fly, cricket, mealworm, midge larvae, mosquito)
• High quality DNA from fresh, frozen, dried or ethanol preserved specimen samples
• Bead Tubes included for difficult-to-lyse insects (due to exoskeletons and reinforced cell walls)
• Efficient lysis – Compatible with the most common disruption devices
• Typical downstream applications: multiplex PCR, Southern blotting, enzymatic reactions

Specifications

• Content And Storage: Room temperature (18 - 25 °C)
• Format: Mini Spin Column
• Isolation Technology: Silica MembraneTechnology and Mechanical (Bead Tube) Disruption
• Sample Type: Insect or Crustacean Specimens (Fresh, Frozen, Dried or Ethanol Preserved)
• Efficiency: Binding capacity: 60 μg
• Elution Volume: Elution volume: 25 - 200 μL
• For Use With (Equipment): Disruption device (e.g. Vortex-Genie 2 with MN Bead Tube Holder), Centrifuge, Vortex mixer
• For Use With (Application): Isolation of genomic DNA from insects and crustaceans
• Includes: NucleoSpin™ DNA Insect Columns, NucleoSpin™ Bead Tubes Type D, Collection Tubes (2 mL), Buffers
• No. of Reactions: 10
• Purity: A₂₆₀/A₂₈₀: 1.7 - 1.9
• Sample Volume: <40 mg fresh, frozen, dried or ethanol preserved insect or crustacean samples
• Size: Fragment size: 200 bp - approx. 50 kbp
• Test Time: 35 min/6 prep
• Yield: <25 μg