Macherey-Nagel™ NucleoSpin™ Blood L, Midi kit for DNA from blood using vacuum processing

Product Code.	Quantity	unitSize
15340195	24 preps	24 preps
15845224	100 preps	100 reactions

Product Code. 15340195 Supplier Macherey-Nagel™ Supplier No. 740954.24

Description

Complete kit with silica membrane midi spin columns for rapid medium-scale purification of genomic DNA from blood and biological samples.

NucleoSpin™ Blood L

• Ready-to-use DNA in less than 60 min
• Complete removal of PCR inhibitors
• Consistently high DNA yields
• Suitable for whole blood (human or animal blood, fresh or frozen)
  • DNA from whole blood treated with citrate, EDTA, heparin, CPDA
  • DNA from serum, plasma, buffy coat, platelets, body fluids (e.g., amniotic fluid)
  • DNA from cultured cells
• Typical downstream applications: PCR, Southern blotting, enzymatic reactions

Specifications

• Color: White band
• Concentration: 200 to 500 ng/μL
• Content And Storage: Columns: 4 °C; Other components: Room temperature (18-25 °C)
• Format: Midi Spin Column
• Isolation Technology: Silica Membrane Technology
• Sample Type: Cell Cultures, Whole Blood
• Efficiency: Binding capacity: 250 μg
• Elution Volume: Elution volume: 120 - 200 μL
• Final Product Type: Genomic DNA
• For Use With (Equipment): Centrifuge
• For Use With (Application): Medium scale purification of DNA from blood
• Includes: NucleoSpin Blood L Columns with Collection Tubes, Collection Tubes (15 mL), buffers, Proteinase K
• No. of Reactions: 24 Preps
• Quantity: 24 preps
• Purity: A₂₆₀/A₂₈₀: 1.6 - 1.9
• Sample Volume: 0.2 - 2 mL blood, 2 x 10⁷ cells
• Test Time: 60 min/prep
• Yield: 40 - 60 μg (2 mL whole blood)

Safety and Handling

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 4
• Respiratory sensitiser Category 1
• Serious eye damage/eye irritation Category 2
• Skin sensitiser Category 1
• Flammable liquid Category 3
• Skin corrosion/irritation Category 2
• Specific target organ toxicity Category 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H336-May cause drowsiness or dizziness.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P284-¢In case of inadequate ventilation! wear respiratory protection.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contain: Proteinase k, propan-2-ol, guanidine hydrochloride, ethanol