Macherey-Nagel™ NucleoSpin™ 96 Virus, 96-well kit for viral RNA/DNA purification

Product Code. 12796533

Brand: Macherey-Nagel™ 740691.2

Description

Time saving parallel isolation of viral RNA / DNA from biological fluids in proven 96‐well plate format.

Designed for the simultaneous purification of viral RNA and DNA. The kit combines the selectivity of well established silica membrane binding of nucleic acids with high throughput 96-well format. With the NucleoSpin™ 96 Virus method, RNA viruses are quickly and efficiently lysed by Lysis Buffer RAV1 which is a highly concentrated GITC solution. Compared to RNA viruses, DNA viruses (e.g., HBV) are usually more difficult to isolate and require a digestion of samples with Proteinase K which is provided in the kit. Lysis buffer and ethanol create appropriate conditions for binding of nucleic acids to the silica membrane of the NucleoSpin™ Virus Binding Plate. Carrier RNA included in Lysis Buffer RAV1 improves binding and recovery of low concentrated viral RNA/DNA. Contaminations (potential PCR inhibitors) like salts, metabolites, and soluble macromolecular cellular components are removed in washing steps with ethanolic Wash Buffer RAW and Buffer RAV3. The purified viral nucleic acids can be eluted in low salt buffer or water and are ready-to-use in subsequent downstream applications like RT-PCR or PCR.

• Silica membrane technology
• Sample material: Cell-free biological fluid, Plasma, Serum
• Fragment size: 100 bp to 50 kbp
• Theoretical binding capacity: 40 μL
• Elution volume: 70 to 100 μL
• Preparation time: 60 min./plate

Specifications

• Content And Storage: 18°C to 25°C
• Format: 96-well Plate
• For Use With (Application): Isolation of viral nucleic acids
• No. of Reactions: 192 Preps
• Sample Volume: <150 μL
• Target: Viral RNA and DNA

Safety and Handling

Signal Word

• Danger

Hazard Category

• Respiratory sensitiser Category 1
• Skin corrosion/irritation Category 2
• Flammable liquid Category 3
• Acute toxicity Category 4
• Serious eye damage/eye irritation Category 2
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H226-Flammable liquid and vapour.
• H302-Harmful if swallowed.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
• H412-Harmful to aquatic life with long lasting effects.

Precautionary Statement

• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P273-Avoid release to the environment.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .