Macherey-Nagel™ Nucleospin™ 96 Plasmid Transfection-grade

A fast way to purify transfection-grade plasmid DNA using mini-format technology to reduce endotoxins from bacterial lysates. Full and Core kits utilize a 96-well plate format for an efficient high-throughput process.

Product Code	Brand	No. of Samples	Product Type	Includes
15553636	Macherey-Nagel™ 740492.4	4 x 96	Core Kit	Filter Plates, Binding Plates, Buffers, RNase A
15543636	Macherey-Nagel™ 740492.24	24 x 96	Core Kit	Filter Plates, Binding Plates, Buffers, RNase A
15533636	Macherey-Nagel™ 740491.4	4 x 96	Full Kit	Filter Plates, Binding Plates, MN Wash Plates, Culture Plates, Elution Plates, Buffers, RNase A

Description

Endotoxins are co-purified during typical plasmid preparations from bacterial lysates. Since they interfere with eukaryotic cell survival, endotoxin reduction is essential prior to cell transfection. NucleoSpin™ Plasmid Transfection-grade kits are designed to reduce endotoxins from bacterial lysates.

• Purifies high-copy plasmids for transfection
• Novel technology to diminish endotoxin content (< 50 EU/μg DNA) for successful transfection
• Rapid purification from E. coli via automatic, manual, vacuum or centrifugation processing
• Ultra-fast procedure simplifies the work-flow
• High-throughput — 96-well plates allow for easy automation and supports plasmid screening
• Downstream applications: transfection of common cell lines (lipofection, electroporation)
• Also suitable for standard molecular biology application, such as cloning and sequencing

Specifications

• Bacterial Endotoxins: <50 EU/ng DNA
• Format: 96-well Plate
• Sample Type: Bacterial Culture (E. coli, High-copy Plasmids)
• Elution Volume: Elution Volume: 100 - 200 μL
• For Use With (Equipment): Centrifuge, Vacuum manifold (e.g. NucleoVac 96)
• Purity: A₂₆₀/A₂₈₀: 1.80 - 1.85
• Size: < 15 kbp
• Yield: 5 - 20 μg

• Content And Storage: Room temperature (18 - 25 °C)
• Isolation Technology: Silica Membrane and Endotoxin-reduction Technology
• Efficiency: Binding capacity: 20 μg
• Final Product Type: Transfection-grade plasmid DNA
• For Use With (Application): Rapid purification of plasmids for transfection
• Sample Volume: Up to 5 mL
• Test Time: 45 min/plate