Macherey-Nagel™ NucleoSpin™ 96 Plant II

Designed for the isolation of genomic DNA from plant tissue

Product Code	Brand	No. of Reactions
12763560	Macherey-Nagel™ 740663.2	2 x 96 Preps
12773560	Macherey-Nagel™ 740663.24	24 x 96 Preps

Description

NucleoSpin™ 8/96 Plant is designed for the isolation of genomic DNA from plant tissue (e.g., Arabidopsis, tobacco or maize). Depending on the individual sample (20mg to 100mg) typical yields are in the range from 5 to 30μg DNA. For RNA-rich samples, it is recommended to add RNase to the lysis buffer or perform a subsequent RNase treatment. The general isolation procedure ensures lysis of plant material with a CTAB lysis buffer developed especially for plant samples. CTAB is removed completely in the subsequent washing steps and thus does not interfere with downstream PCR*. The binding process is reversible and specific for nucleic acids. Contaminations are removed by washing. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer. The obtained DNA can be used directly for PCR*, Southern blotting or any kind of enzymatic reaction. NucleoSpin™ 8/96 Plant can be used in a centrifuge with deep buckets allowing processing of NucleoSpin™ 8 well strips/96 well plates stacked on 96 well blocks. NucleoSpin™ 8/96 can also be used under vacuum, manually in the NucleoVac 96, or automated on many common liquid handling instruments. Up to 192 samples (two full 96 well plates) can be processed in 2 to 3hrs depending on equipment used for pipetting and homogenisation.
Required hardware
Centrifugation
For centrifuge processing of NucleoSpin 8/96 plant kits a microtiterplate centrifuge is required which is able to accommodate the NucleoSpin Plant strips/plate stacked on a round or square well block or rack with tube strips and reaches accelerations of 5, 600xg to 6, 000xg (bucket height: 85mm). For further information on suitable centrifuges please contact our Technical Service team. For centrifuge processing of the 8 well Strips, the reusable Starter Set C, containing column holders C, dummy strips, MN square well blocks, and tube strips, is required.
Vacuum processing
The NucleoSpin 8/96 plant kits can be used manually with the NucleoVac 96 vacuum manifold. Additionally, a suitable centrifuge for sample preparation steps may be required. For vacuum processing of the 8 well strips the reusable Starter Set A, containing column holders A and dummy strips is required. For automation on laboratory platforms with standard 96 well plate vacuum chambers the use of the Starter Set A is also required.
Kit components
NucleoSpin™ 8 Plant - NucleoSpin™ plant binding strips, wash plates, round well blocks, racks with tube strips, cap strips, gas permeable foil, self-adhering foil, buffers, RNase A. NucleoSpin™ 96 Plant - NucleoSpin™ plant binding plates, square well blocks, racks with tube strips, cap strips, gas-permeable foil, buffers, RNase A.

• Silica membrane technology
• Two formats: 8 well strip or 96 well plates
• Yield: 5μg to 30μg
• Sample material: 20μg to 100μg plant tissue or cells
• Elution volume: 100μL to 200μL
• Binding capacity: 25μg (8 well format)/30μg (96 well format)
• Preparation time: 120min for 12 strips/one 96 well plate
• MN wash plate minimises cross-contamination For isolation of genomic DNA from plant cells/tissue in a 8 well strip format or 96 well format.

Specifications

• Product Type: 96 Plant II