Macherey-Nagel™ NucleoSpin™ 96 PCR Clean-up, 96-well kit for PCR clean up

Product Code. 12755214

Brand: Macherey-Nagel™ 740658.4

Description

NucleoSpin™ 96 Gel and PCR Clean-Up Kit provides time saving medium to high throughput PCR clean up.

The NucleoSpin™ 96 PCR Clean-up kit allows direct clean-up of PCR reaction mixtures. Within the procedure the addition of chaotropic salt (Buffer NT) allows a reversible adsorption of the PCR products to the silica membrane of the NucleoSpin™ 96 PCR Clean-up Binding Plates. High purity of the PCR-products is achieved by complete removal of primers, primer-dimers, salts, nucleotides, and proteins (e.g., polymerases, BSA) in subsequent washing steps using Buffer NT3. Highly pure PCR products are finally eluted with Elution Buffer NE (5 mM Tris/HCl, pH 8.5) or water (pH 8.5), and can be used directly for further applications.

• Silica membrane technology
• Processing: manual/automated, vacuum/centrifugation
• Sample material: < 10 0μL PCR reaction mixture
• Elution volume: 75 to 150 μL
• Binding capacity: 15 μg
• Preparation time: 45 min./plate
• Fragment size: 65 bp to 10 kb

Specifications

• Content And Storage: 18°C to 25°C
• Format: 96-well Plate
• For Use With (Application): PCR clean up
• No. of Reactions: 384 Preps
• Sample Volume: <100 μL

Safety and Handling

Signal Word

• Warning

Hazard Category

• Acute toxicity Category 4
• LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement

• H302-Harmful if swallowed.
• H412-Harmful to aquatic life with long lasting effects.

Supplemental information

• MIXTURE LIST-Contains : guanidinium thiocyanate