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Macherey-Nagel™ NucleoSpin™ 8 Blood, 8-well kit for DNA from blood
Description
After lysis of blood cells genomic DNA is selectively bound to a silica membrane while contaminants are washed away effectively. Elution is possible even under vacuum without cross-contamination. Due to its plate design and the possibility to perform the complete procedure under vacuum, NucleoSpin™ 8 Blood can be easily adapted to common liquid handling instruments for fully automated processing without manual interactions.
Kit components: NucleoSpin™ Blood binding strips, MN wash plates, MN tube strips, buffers, proteinase K.
Required hardware:
Vacuum processing - the NucleoSpin 8 Blood kits can be used manually with the NucleoVac 96 vacuum manifold. For vacuum processing of the 8-well Strips the reusable Starter Set A, containing Column Holders A and Dummy Strips is required- Silica membrane technology
- Yield: 4μg to 6μg
- Sample material: ≤200μL whole blood, 2 x 106 cultured cells
- Elution volume: 100μL
- Binding capacity: 20μg
- Preparation time: 35min
- Fragment size: 300bp to 30kb
Specifications
Specifications
| Format | 8-well Strip |
| Isolation Technology | Silica Membrane Technology |
| For Use With (Application) | For Manual and Automated Isolation of Genomic DNA |
| No. of Reactions | 60 x 8 |
| Product Type | NucleoSpin™ 8 Blood Binding Strip |
| Test Time | 35min. |
| Yield | 4 to 6μg |
Safety and Handling
- Flammable liquid Category 3
- Acute toxicity Category 4
- Respiratory sensitiser Category 1
- Serious eye damage/eye irritation Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity Category 3
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