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Macherey-Nagel Bioanalysis™ NucleoMag 96 PCR Kit with Magnetic Beads
Description
The NucleoMag 96 PCR procedure is based on reversible adsorption of nucleic acids to paramagnetic beads under appropriate buffer conditions. Adjusting the PCR reaction to binding conditions and addition of paramagnetic beads can be carried out simultaneously. After magnetic separation and removal of supernatant, the beads are washed to remove contaminants and salt. A short drying step is necessary to remove ethanol from previous washing steps. Finally, highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The NucleoMag 96 PCR kit can be used either manually or automated on standard liquid handling instruments.
- Magnetic bead technology
- Sample Amount: < 50 μL
- Fragment Size: 150 bp to 10 kbp
- Typical Recovery: 80 to 95 %
- Binding Capacity: 0.3 μg/μL beads
- Preparation Time: 40 to 120 min/96 preps
- Storage: 18 to 25°C
Specifications
Specifications
| Sample Type | PCR Reaction Mixture |
| Elution Volume (Metric) | 25 to 100 μL |
| For Use With (Application) | PCR clean up |
| Quantity | 384 |
Safety and Handling
- Warning
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- H302-Harmful if swallowed.
- H319-Causes serious eye irritation.
- MIXTURE LIST-Contains : guanidine hydrochloride
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