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Macherey-Nagel™ NucleoBond™ RNA Soil Midi kit for RNA from soil

Product Code. 15825224
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20 Preps
This item is not returnable. View return policy

Product Code. 15825224

Brand: Macherey-Nagel™ 740140.20

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This item is not returnable. View return policy

Description

Easy handling and superior speed for metagenomic soil analysis, MN Bead Tubes Type A included.

NucleoBond™ RNA Soil kit enables efficient isolation of high quality RNA and DNA from any kind of soil or sediment sample. Most soils contain relatively low amounts of RNA, compared to DNA. Therefore, the kit is designed with proven NucleoBond™ technology to enable high sample input and and pure nucleic acids.

  • Anion exchange technology to optimize RNA yield and purity—suitable for metagenomic studies
  • Combination of mechanical homogenization and chemical lysis allows processing of large samples
  • Parallel preparation of RNA and DNA in one hour*
  • Sample disruption is achieved with NucleoSpin™ Bead Tubes Type A, optimized Lysis Buffer, and Phenol:Chloroform:Isoamylalcohol
  • Optional Buffer OPT enables RNA and DNA purification from mineralic and organic soil samples

* DNA isolation requires DNA Set for NucleoBond™ RNA Soil, sold separately (man. part no. 740141.20)

Specifications

Format Gravity Flow Column
Isolation Technology Anion Exchange Chromatography, Gravity Flow Columns, NucleoSpin Bead Tubes
Elution Volume Elution volume: 100 μL
Sample Type Sediment, Soil
Efficiency Binding capacity: 600 μg
For Use With (Equipment) Centrifuge for microcentrifuge tubes
For Use With (Application) RNA isolation from soil for metagenomic study
Includes NucleoBond Soil Columns, NucleoSpin Bead Tubes Type A, NucleoSpin Finisher, buffers
No. of Reactions 20
Purity A260/A280: 1.7 - 2.1
Sample Size <2 g
Test Sensitivity RNA integrity number (RIN): > 8.5
Test Time 60 min. per 12 preps
Yield 1 - 10 μg
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Safety and Handling

compliance-icons
Product Identifier
  • NucleoBond RNA Soil (20)
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
 Supplemental information
  • MIXTURE LIST-Contains : guanidium thiocyanide, ethanol
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