Macherey-Nagel™ NucleoBond™ PC 500, Maxi Kit for Transfection-grade Plasmid DNA

Product Code.	No. of Reactions	unitSize
12840731	10 Preps	10 reactions
12790941	100 Preps	100 reactions
12748482	25 Preps	25 reactions
12823841	50 Preps	50 reactions

Product Code. 12840731 Supplier Macherey-Nagel™ Supplier No. 740574

Description

Recover and purify plasmid DNA without degredation. NucleoBond™ PC 500 Columns use a silica-based anion exchange technology to concentrate and purify transfection-grade DNA.

Features

• The classic anion exchanger for purification of transfection-grade plasmid DNA
• Fast and easy clarification of lysates with NucleoBond™ Folded Filters, no centrifugation required, no shearing forces
• NucleoBond™ Finalizers available as separate tool to avoid time consuming centrifugation for plasmid precipitation
• Proven anion-exchange technology – transfection-grade plasmid DNA
• Separate binding columns of NucleoBond™ PC kits available as NucleoBond™ AX columns

Applications:

• High-copy plasmids from E. coli
• Low-copy plasmids from E. coli (increased buffer volumes required)

Specifications

• Format: Maxi
• Isolation Technology: Anion Exchange Chromatography Technology
• Final Product Type: Columns PC 500
• For Use With (Application): High-copy/low-copy plasmids from E. coli
• Includes: AX 500 Column, Buffers, RNase A, NucleoBond Folded Filters XL
• No. of Reactions: 10 Preps
• Product Type: Anion exchange column
• Sample Volume: 30–150mL (HC)
  100–500mL (LC)
• Size: <300 kbp
• Test Time: Prep time: 80–90 min./4 preps
• Yield: 400 to 500μg

Safety and Handling

Signal Word

• Danger

Hazard Category

• Flammable liquid Category 3
• Skin corrosion/irritation Category 2
• Serious eye damage/eye irritation Category 2
• Respiratory sensitiser Category 1

Hazard Statement

• H226-Flammable liquid and vapour.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
• P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
• P280-Wear protective gloves/protective clothing/eye protection/face protection.

Supplemental information

• MIXTURE LIST-Contain: ethanol (diluted < 20 %)/sodium hydroxide solution (diluted< 2 %)/dodecyl sulfate, sodium salt