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  5. Macherey-Nagel™ NucleoBond™ CB 20/CB 100, Midi kit for high integrity DNA from cells and blood

Macherey-Nagel™ NucleoBond™ CB 20/CB 100, Midi kit for high integrity DNA from cells and blood

Product Code. 11922452
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Yield:
100 μg
5-20 μg
Column Type:
NucleoBond AXG 100
NucleoBond AXG 20
Unit Size:
20 reactions
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Product Code. 11922452

Brand: Macherey-Nagel™ 740507

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This item is not returnable. View return policy

Description

Anion exchange chromatography for purification of DNA from whole blood and cultured cells.

Genomic DNA quality is extraordinarily high. Samples are first lysed under special conditions for removal of erythrocytes. After incubation with proteinase K, the sample is diluted with buffer N2 before loading onto the NucleoBond™ column. After elution with high-salt buffer the genomic DNA is precipitated with isopropanol. The DNA purified by this method is suitable for all kinds of subsequent reactions. Kit components: NucleoBond™ CB 100 kit - NucleoBond AXG 100 columns, buffers, proteinase K. NucleoBond™ CB 20 kit - NucleoBond™ AXG 20 columns, buffers, proteinase K.
  • Anion exchange technology
  • Format: gravity flow columns
  • Sample material: whole blood, buffy coat, cultured cells (up to 20 mL blood)
  • Fragment size: 500bp to 300kb
  • Yield: 100μg or 5-20μg depending on column used
  • Binding capacity: 100μg or 5-20μg depending on column used
  • Preparation time: 4 to 5hrs For purification of up to 500μg genomic DNA from whole blood.

Specifications

Column Type NucleoBond AXG 20
Format Mini Gravity-flow Column
Isolation Technology Anion Exchange Chromatography Technology
For Use With (Application) Applications: Genomic DNA from whole blood, serum, plasma, cultured cells, buffy coat.
No. of Reactions 20 preps
Product Type NucleoBond™ CB
Sample Volume 0.1-1 mL whole blood or plasma, <50 μL buffy coat, 5 x 106 cultured cells
Size 500kbp
Test Time 4-5hr.
Yield 5-20 μg

Safety and Handling

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Hazard Category
  • Respiratory sensitiser Category 1
  • Skin sensitiser Category 1
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