Macherey Nagel™ NTB Binding Buffer


Overview
Brand: Macherey Nagel™

Manufacturer Part Number: 740595.1

1 LT WASH BUFFER NTB 1L

UNSPSC: 41116010

Code: 86

Additional Details:
Additional Details: CAS Number: 50-01-1 Weight: 1.06000kg Transport: UN number : 1993 Chem class : 1 Pack group : III


Quantity 1000mL

Product Code. 12788482

Quantity Price
1 £ 155.0 / 1000mL
Estimated Shipment
10-03-2017
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Description and Specification

Specification

Buffers Wash Buffer NTB
Quantity 1000mL
For Use With For Clean-up

NucleoTrap™ is a silica matrix based kit for isolation of highly pure DNA fragments from agarose gels and aqueous solutions (desalination). The kit can also be used for the removal of nucleotides and/or biotin after labelling reactions as well as for the removal of enzymes. By means of a specially activated spherical silica matrix it is possible to extract DNA fragments from agarose gels or solvents in a very mild manner, without the use of organic solvents. Nucleic acids are bound to the NucleoTrap™ matrix in the presence of a chaotropic salt. The hydrate shell of the nucleic acids is destroyed by this binding buffer, thus facilitating adsorption to the specially treated NucleoTrap™ matrix. A washing step is followed by the elution of DNA with low-salt elution buffer or distilled water. Kit components: NucleoTrap™ suspension, buffers.
  • Silica matrix technology
  • Fragment size: 20bp to 20kb
  • Sample material: 20mg to 600mg agarose gel
  • Elution volume: 20μL to 50μL
  • Binding capacity: 6μg/10μL suspension
  • Typical Recovery: 50% to 90%
  • Preparation time: 60min/6 preps
  • Format: silica bead suspension For extraction of DNA from agarose gels, suitable, for example, for subsequent cloning sequencing, PCR, restriction analysis. Support protocol for concentration and desalination of reaction mixtures.

Documentation

Technical documents