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Macherey Nagel™ NTB Binding Buffer

Brand:  Macherey Nagel™ 740595.1

UNSPSC : 41116010

Code : 86

Additional Details : CAS Number : 50-01-1 Weight : 1.06000kg Transport : UN number : 1993 Chem class : 1 Pack group : III


Quantity 1000mL

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Product Code. 12788482

  • £155.00 / 1000mL


Estimated Shipment
15-08-2017
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Specifications

Description

NucleoTrap™ is a silica matrix based kit for isolation of highly pure DNA fragments from agarose gels and aqueous solutions (desalination). The kit can also be used for the removal of nucleotides and/or biotin after labelling reactions as well as for the removal of enzymes. By means of a specially activated spherical silica matrix it is possible to extract DNA fragments from agarose gels or solvents in a very mild manner, without the use of organic solvents. Nucleic acids are bound to the NucleoTrap™ matrix in the presence of a chaotropic salt. The hydrate shell of the nucleic acids is destroyed by this binding buffer, thus facilitating adsorption to the specially treated NucleoTrap™ matrix. A washing step is followed by the elution of DNA with low-salt elution buffer or distilled water. Kit components: NucleoTrap™ suspension, buffers.
  • Silica matrix technology
  • Fragment size: 20bp to 20kb
  • Sample material: 20mg to 600mg agarose gel
  • Elution volume: 20μL to 50μL
  • Binding capacity: 6μg/10μL suspension
  • Typical Recovery: 50% to 90%
  • Preparation time: 60min/6 preps
  • Format: silica bead suspension For extraction of DNA from agarose gels, suitable, for example, for subsequent cloning sequencing, PCR, restriction analysis. Support protocol for concentration and desalination of reaction mixtures.
Description & Specifications

Specifications

For Clean-up
Wash Buffer NTB
1000mL

Safety and Handling

Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Serious eye damage/eye irritation Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H226-Flammable liquid and vapour.
  • H302-Harmful if swallowed.
  • H319-Causes serious eye irritation.
  • H336-May cause drowsiness or dizziness.

Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P233-Keep container tightly closed.
  • P264-Wash thoroughly after handling.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P330-Rinse mouth.
  • P337+P313-If eye irritation persists: Get medical advice/attention.
  • P370+P378-In case of fire: Use to extinguish.
  • P403+P235-Store in a well-ventilated place. Keep cool.

Documentation

Technical Documents