The NucleoSpin™ Extract II procedure is the easiest way to purify DNA fragments from agarose gels as well as for direct purification of PCR products. The purification procedure from enzymatic reactions (eg. PCR) allows fast and easy removal of enzymes, nucleotides, salts and other impurities. The NucleoSpin™ Extract II columns provide convenient performance for PCR clean-up. After addition of binding buffer NT the mixture is applied onto the silica membrane. Contaminations are removed by a simple washing step with ethanolic buffer NT3.Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline buffer NE (5mM Tris-HCl, pH8.5). For DNA extraction from agarose gels the agarose gel slice is incubated with high-salt buffer and applied to a NucleoSpin™ Extract II column. After centrifugation and subsequent washing the DNA can be eluted under low ionic strength conditions with slightly alkaline buffer NE (5mM Tris-HCl, pH8.5). Kit components: NucleoSpin™ Extract II columns, collecting tubes 2mL, buffers.
Silica membrane technology
Yield: 70% to 95%
Sample material: <400μL PCR reaction mixture; <400mg TAE/TBE agarose gel
High recovery of fragments 65bp to 10kb
Elution volume: 15μL to 50μL
Binding capacity: 25μL
Preparation time: 10min/6 preps
Format: mini spin column For direct purification of PCR* products and extraction of DNA from agarose gels.