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Macherey-Nagel™ Binding Buffer NT2

Product Code. 10144951
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100 mL
Unit Size:
100mL
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Product Code. 10144951 Supplier Macherey-Nagel™ Supplier No. 740597

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Description

Binding Buffer NT2 to be used with NucleoTrap or NucleoTraPCR kits.

NucleoTrap™ is a silica matrix based kit for isolation of highly pure DNA fragments from agarose gels and aqueous solutions (desalination). The kit can also be used for the removal of nucleotides and/or biotin after labelling reactions as well as for the removal of enzymes. By means of a specially activated spherical silica matrix it is possible to extract DNA fragments from agarose gels or solvents in a very mild manner, without the use of organic solvents. Nucleic acids are bound to the NucleoTrap™ matrix in the presence of a chaotropic salt. The hydrate shell of the nucleic acids is destroyed by this binding buffer, thus facilitating adsorption to the specially treated NucleoTrap™ matrix. A washing step is followed by the elution of DNA with low-salt elution buffer or distilled water. Kit components: NucleoTrap™ suspension, buffers.
  • Silica matrix technology
  • Fragment size: 20bp to 20kb
  • Sample material: 20mg to 600mg agarose gel
  • Elution volume: 20μL to 50μL
  • Binding capacity: 6μg/10μL suspension
  • Typical Recovery: 50% to 90%
  • Preparation time: 60 min./6 preps
  • Format: silica bead suspension For extraction of DNA from agarose gels, suitable, for example, for subsequent cloning sequencing, PCR, restriction analysis. Support protocol for concentration and desalination of reaction mixtures.

Specifications

Chemical Name or Material Binding Buffer NT2
For Use With (Application) For Clean-up
Quantity 100 mL
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