Invitrogen™ NSFL1C Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA521633

Product Code. 13237508

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain. Predicted reactivity: Mouse (96%), Rat (96%), Xenopus laevis (83%), Pig (98%), Chicken (87%), Rhesus Monkey (100%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

p47, also known as NSFL1C, UBX1, UBXD10 or UBXN2C, is a 370 amino acid protein that localizes to both the nucleus and the golgi apparatus (specifically to golgi stacks) and contains one SEP domain and one UBX domain. Functioning as part of a ternary complex with VCP (a protein involved in the heterotypic fusion of transport vesicles with their target membranes) and Syntaxin 5, p47 interacts with and reduces the ATPase activity of VCP and is required for the fragmentation of golgi stacks during mitosis and for subsequent reassembly of golgi stacks after mitosis. p47 is subject to phosphorylation during mitosis, which inhibits p47-golgi interaction and is, therefore, required for proper golgi stack formation and cisternal regrowth. Human p47 shares 89% sequence identity with its mouse counterpart, suggesting a conserved role between species. Multiple isoforms of p47 exist due to alternative splicing events.

Specifications

• Antigen: NSFL1C
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Nsfl1c
• Gene Alias: dJ776F14.1; Munc-18c; NSFL1 (p97) cofactor (p47); NSFL1 cofactor; NSFL1 cofactor p47; NSFL1C; p47; p97 cofactor p47; RP4-776F14.2; SHP1 homolog; Stxbp3a; UBX domain-containing protein 2C; UBX1; UBXD10; UBXN2C; XY body-associated protein XY40; XY40
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 386649, 55968, 83809
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: O35987, Q9CZ44, Q9UNZ2
• Gene Symbols: Nsfl1c
• Immunogen: Recombinant protein encompassing a sequence within the center region of human NSFL1C. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG