Invitrogen™ NSE Polyclonal Antibody

Product Code.	Quantity	unitSize
11515862	100 μL	100µL

Product Code. 11515862 Supplier Invitrogen™ Supplier No. PA110010

Description

Chicken Polyclonal Antibody

PA1-10010 can be used to trace NSE and to identify neuronal cells in cell culture and sectioned material.

Neuron specific enolase (NSE, ENO1, ENO2, ENO3) is an enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and the reverse reaction in gluconeogenesis. NSE has a high stability in biological fluids and can easily diffuse to the extracellular medium and cerebrospinal fluid (CSF) when neuronal membranes are injured.NSE is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The alpha-subunit is expressed in most tissues, the beta-subunit only in muscle, and the gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Co-expression of NSE and chromogranin A is common in neuroendocrine neoplasms. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to NSE protein are useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury. NSE is used clinically as a sensitive and useful marker of neuronal damage in several neurological disorders including stroke, hypoxic brain damage, status epilepticus, Creutzfeldt-Jakob disease, and herpetic encephalitis. Further, NSE is found in elevated concentrations in plasma and certain neoplasias that include pediatric neuroblastoma and small cell lung cancer.

Specifications

• Antigen: NSE
• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: Conc. Not Determined
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% sodium azide
• Gene: ENO2
• Gene Accession No.: P07323, P09104, P17183
• Gene Alias: 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; AI837106; D6Ertd375e; Eno; ENO2; Eno-2; ENOG; Enolase 2; enolase 2 (gamma, neuronal); enolase 2, gamma neuronal; enolase 2, gamma, neuronal; epididymis secretory protein Li 279; gamma enolase; gamma-enolase; gamma-enolase-like; gamma-subunit of enolase; HEL-S-279; LOC100911625; Neural enolase; neuron specific enolase; neuron specific gamma enolase; Neuronal Enolase; neuronal enriched enolase; neurone-specific enolase; neuron-specific enolase; NSE; RNEN3
• Gene Symbols: ENO2
• Host Species: Chicken
• Immunogen: Recombinant full length human NSE expressed in and purified from E. coli.
• Purification Method: PEG precipitation
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 13807, 2026, 24334
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgY