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  5. Cytiva nProtein A Sepharose™ 4 Fast Flow Affinity Media

Cytiva nProtein A Sepharose™ 4 Fast Flow Affinity Media

Product Code. 11359931 Shop All Cytiva Products
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Quantity:
5 mL
25 mL
pH Range:
2 to 10
3 to 9
Unit Size:
25mL
5mL
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Product Code. Quantity pH Range unitSize
11359931 5 mL 3 to 9 5mL
11379931 25 mL 2 to 10 25mL
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Product Code. 11359931 Supplier Cytiva Supplier No. 17528001

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Description

Used for purification of monoclonal and polyclonal antibodies. Cytiva Lifescience™ nProtein A Sepharose™ 4 Fast Flow Affinity Media contains native protein A coupled to Sepharose 4 Fast Flow.

  • Low leakage of protein A with nearly twice total IgG binding capacity of Protein A Sepharose CL-4B
  • Used in large-scale FDA-approved processes
  • Manufactured without using animal-derived components
  • Rigidity and high degree of substitution of Sepharose 4 Fast Flow matrix facilitates speedy process of large volumes dilute cell culture fluid
  • High chemical, thermal and mechanical stability
  • Withstands high concentrations of hydrogen bond disrupting agents
TRUSTED_SUSTAINABILITY

Specifications

Quantity 5 mL
Stability No significant change in chromatographic performance after 1 week storage using 8 M Urea, 6 M Gua-HCI, 2% Benzyl Alcohol or 20% Ethanol
For Use With (Application) Purification of Monoclonal and Polyclonal Antibodies
Storage Requirements 2°C to 8°C
Binding Capacity >35 mg Human IgG/mL
Product Type nProtein A Sepharose 4 Fast Flow Media
Flow Rate 150 to 250 cm/Hoμr
Particle Size 90 μm
Height (English) Bed 9.84 in.
Height (Metric) Bed 25 cm
Max. Pressure 100 kPa
Matrix 4% Cross-Linked Agarose
pH Range 3 to 9
Storage Buffer 20% Ethanol
Ligand Type Native Protein A (S. aureus)
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