Invitrogen™ Novex™ TNF-α Human ELISA Kit

Each ready-to-use kit is validated for sensitivity, specificity, precision, and lot-to-lot consistency

Brand: Invitrogen™

Manufacturer Part Number: KHC3011

HU TNF ALPHA ELISA KITThe TNF- Human ELISA Kit quantifies natural and

UNSPSC: 12352203

Code: 50

Additional Details:
Additional Details: Weight: 0.56000kg

Product Code. 10737663

Quantity Price
1 £ 352.0 / 96 tests
EU Stock 9
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For Research Use Only. All usage must comply with product instructions.

Description and Specification


Accession Number NM_000594.2, NP_000585.2
For Use With (Application) Colorimetric Detection
Gene ID (Entrez) 7124
Includes Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers
Incubation Time 4 hr.
For Use With (Equipment) Microplate Reader
Marker Gene TNF-α
Quantity 96 assays
Sensitivity <1.7pg/mL
Sample Type Plasma, Serum, Supernatants
Sample Size 100μL
Target tumor necrosis factor

Research use only kit designed to detect and quantify the level of human TNF-α in serum, plasma, and supernatant using 96-well plates and a microplate reader.

Easy-to-Run Sandwich ELISA
A monoclonal capture antibody specific for Human TNF-α has been machine coated onto the wells of the 96-well plate, ensuring low well-to-well variability and eliminating the need for overnight coating. Samples, including a standard of known Human TNF-α content, controls, and unknowns, are pipetted into these wells. During the first incubation, Human TNF-α antigen binds to the immobilized capture antibody on one site. After washing, a biotinylated monoclonal antibody specific for Human TNF-α is added. During the second incubation, this antibody binds to the immobilized Human TNF-α antigen at a different site. After washing, a streptavidin-horseradish peroxidase (HRP) is added. This binds to the biotinylated detection antibody to complete the four–member sandwich. After a second incubation and washing to remove all unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of Human TNF-α present in the original specimen and the optical density can be read on a standard microplate reader. Total assay incubation time is only four hours.

  • Easy to run
  • Rapid protocol
  • Convenient precoated, 96-well plate supplied as removable 8-well strips to accommodate smaller samples run

See protocol insert for more information on validation.

Apoptosis, Apoptosis Related Factors, Cell Analysis, Cell Signaling, Cell Viability, Proliferation & Function, Immunoassays, Ready-To-Use Immunoassay

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