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Invitrogen™ Human SAA ELISA Kit

ELISA

Brand:  Invitrogen™ KHA0011

396.72 GBP valid until 2025-12-31
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Product Code. 10059202

  • £522.00 / 96 tests
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For Research Use Only. All usage must comply with product instructions.
 

Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers
This item is not returnable. View return policy

Description

Description

The Human SAA ELISA Kit quantifies human serum amyloid A in serum, plasma, buffered solution, or cell culture medium. The assay will recognize both the natural 18 kDa isoform and recombinant SAA. Principle of the method The Human SAA solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The serum amyloid A family comprises a number of differentially expressed apolipoproteins, acute-phase SAA1 and SAA2, the former being the major component in plasma and constitutive SAAs. Although the liver is the primary site of synthesis of both SAA types extrahepatic production has been reported. The in vivo concentrations increase by as much as 1000 fold during inflammation. Several studies have expressed its importance in the diagnosis and monitoring of various diseases. Pathological SAA values are often detected in association with normal CRP concentrations; SAA rises earlier and more sharply than CRP. Recently, a broader view of SAA expression and function has been emerging.

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TRUSTED_SUSTAINABILITY
Specifications

Specifications

P0DJI8
4 ng/mL
ELISA
Human
Colorimetric Microplate Reader
PIG4,SAA,SAA2,TP53I4
7.4%
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
SAA (Serum Amyloid A)
RUO
2°C to 8°C
1 hrs 20 mins
9.4 to 600 ng/mL
HRP
Plasma, Serum, Supernatant
Colorimetric Detection
6288
3 hr.
6.1
HRP
96 Tests
Plasma 100 μL, Serum 100 μL, Supernatant 100 μL
SAA
3 hrs
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For Research Use Only. Not for use in diagnostic procedures.