Invitrogen™ Novex™ SAA Human ELISA Kit

Quantifies natural and recombinant human SAA in serum, plasma, buffered solution and tissue culture medium

Brand:  Invitrogen™ KHA0011

Product Code. 10059202

Description

Serum amyloid A (SAA) proteins comprise a family of small (12-14 kDa, 104-112 amino acid residues), differentially expressed proteins that are highly conserved among vertebrates. SAA proteins are involved in the acute phase responses; these are the immediate early host responses to inflammation. During the acute phase, circulating SAA levels are increased by 100-1000 fold, reaching concentrations of up to one milligram per milliliter. Liver is the major site of SAA synthesis, although extrahepatic expression has also been reported. Mouse SAA gene family maps to chromosome 7. In mouse, five SAA genes and four protein products have been identified: mouse SAA1, SAA2, SAA3 are the acute phase isoforms, SAA5 is constitutively expressed, and SAA4 is a pseudogene. SAAs belong to a category of acute phase proteins that also includes C-reactive protein (CRP), haptoglobin, ceruloplasmin, complement components C3 and C4, a1-acid glycoprotein, a1-proteinase inhibitor, and fibrinogen.

Specifications

• Accession Number: P0DJI8
• Conjugate: Biotin
• Gene ID (Entrez): 6288
• Incubation Time: 3 hr.
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Product Type: ELISA Kit
• Regulatory Status: RUO
• Sample Type: Plasma,Serum,Supernatant
• Sample Volume: Plasma,100 μL|Serum,100 μL|Supernatant,100 μL
• Test Time: 1 hr 20 min
• Target: Human
• Interassay CV: 7.4%
• Label or Dye: HRP

• Assay Range: 9.4-600 ng/mL
• For Use With (Application): Colorimetric Detection
• Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers
• For Use With (Equipment): Colorimetric Microplate Reader
• Marker Gene: SAA (Serum Amyloid A)
• Quantity: 96 Tests
• Sensitivity: 4 ng/mL
• Sample Size: 10μL
• Storage Requirements: 2-8°C
• Total Assay Time: 3 hr
• Gene Symbols: SAA1
• Intraassay CV: 6.1%

For Research Use Only. Not for use in diagnostic procedures.