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Invitrogen™ Novex™ IEF Protein Gels, pH 3-7, 1.0 mm
Description
Novex™ pH 3-7 IEF Gels are used for pI determination and are excellent for native applications using soluble proteins. Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI). The pI is the pH at which a protein has no net charge and does not move in an electric field. Vertical IEF gels effectively create a pH gradient so proteins separate according to their unique pI.
IEG gels can be used to readily detect minor changes in a protein due to deamination, phosphorylation, or glycosylation, and can resolve different proteins of similar size that cannot be resolved on standard SDS-PAGE gels. Pre-focusing is not required. Total run time is approximately 2.5 hours.
Formulation
Novex™ IEF gels are 5% polyacrylamide and consist of high-purity acrylamide, bisacrylamide, TEMED, APS, ultrapure water, and 2% ampholytes. They do not contain denaturing reagents.
Recommended buffers
IEF is a sensitive technique which is affected by many factors. Our optimized, premixed IEF buffers reduce variability and help you obtain consistent results.
Specifications
Specifications
| For Use With (Equipment) | Mini Gel Tank, XCell SureLock Mini-Cell |
| Gel Percentage | 5% |
| Gel Size | Mini |
| Gel Thickness | 1.0 mm |
| Gel Type | IEF |
| pH Range | 3.5 to 6.8 |
| Sample Loading Volume | 25 μL |
| Separation Type | Isoelectric Focusing |
| Wells | 10-well |
| Mode of Separation | pI (Charge) |
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