Invitrogen™ MIP-2 (CXCL2) Rat ELISA Kit

Product Code.	Quantity	unitSize
10242403	96 Assays	Each

Product Code. 10242403 Supplier Invitrogen™ Supplier No. KRC1021

Description

Sandwich ELISA Kit

The Rat MIP-2 (CXCL2) ELISA quantitates Rt MIP-2 in human serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt MIP-2. Principle of the method The Rat MIP-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Macrophage inflammatory protein 2 is a chemokine involved in the pulmonary inflammatory response. MIP-2 is produced by activated monocytes and neutrophils and expressed at sites of inflammation. In vitro, it suppresses hematopoietic progenitor cell proliferation.

Specifications

• Accession Number: P30348
• Assay Range: 10-640 pg/mL
• Assay Sensitivity: <1 pg/mL
• Conjugate: Biotin
• Product Type: ELISA Kit
• Sample Type: Serum, Supernatant
• Target Species: Rat
• For Use With (Application): Colorimetric Detection
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 114105
• Gene Symbol: Cxcl2
• Incubation Time: 3.5 hr.
• Interassay CV: 8.6%
• Intraassay CV: 5.9%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Marker Gene: MIP-2
• Quantity: 96 Assays
• Regulatory Status: RUO
• Sample Volume: Serum (50 μL), Supernatants (50 μL)
• Storage Requirements: 2°C to 8°C
• Target: Rat
• Test Time: 1 hr. 20 min.
• Total Assay Time: 3 hr. 30 min.