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Invitrogen™ Luminex™ Cytokine Human 10-Plex Panel
Description
Includes
Contains premixed antibody coated capture beads, standard, premixed detector antibody, diluents, SAV concentrate, buffers, wash solution, filter plate, complete protocol and lot-specific technical data sheet.
The Cytokine Human 10-Plex Panel for the Luminex platform is specifically designed for quantifying human GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10 and TNF-α in serum, plasma, and tissue culture supernatant. Assay may be run alone or in combination with other select Luminex singleplex kits. The panel is suitable for use with the Luminex 100™/200™ and FLEXMAP 3D™ systems.
Comprehensive Human Analyte Panel
The Human Cytokine 10-Plex Panel for the Luminex platform provides reagents for the accurate, reproducible, and sensitive quantitation of human proteins including:
Luminex xMAP™ Technology—Effective and Proven Analytical Tool
The panel is based on xMAP technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex assays. Polystyrene microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. At the completion of the sandwich immunoassay, beads can be read using the Luminex 100™/200™ and FLEXMAP 3D™ detection systems, instruments based on flow cytometry, two lasers, real-time digital signal processing, and xPONENT™ software to distinguish bead color (analyte) and assay signal strength (PE) fluorescence intensity.
More Validation for Confidence in Your Results
The Human Cytokine 10-Plex Panel has been validated using the same rigorous criterion that distinguishes Luminex assays from other commercially available assays. Standard curves generated for each lot of product to assure quantitation consistency. Kit includes product insert that outlines the specifications for each marker in the assay.
- Superior performance: accurate, reproducible, and sensitive quantitation of multiple proteins
- High quality: fully qualified antibodies permit excellent specificity and sensitivity
- Fast and easy protocols: perform your multiplex assay and analyze your data typically in less than one day
Cell Analysis, Cell Signaling, Cell Viability, Proliferation & Function, Immunoassays, Ready-To-Use Immunoassay
Order Info
Shipping Condition: Dry Ice
Specifications
Specifications
| Quantity | 100 Assays |
| Bead Type | Polystyrene |
| Assay Sensitivity | See Product Insert |
| For Use With (Application) | Fluorescent Detection |
| For Use With (Equipment) | Luminex Instruments |
| Gene ID (Entrez) | 1437, 3438, 3553, 3558, 3565, 3567, 3569, 3576, 3586, 7124 |
| Protein Subtype | GM-CSF, IFN-γ, IL-1β, IL-10, IL-2, IL-4, IL-5, IL-6, IL-8, TNF-α |
| Sample Type | Cell Culture Supernatant, Plasma, Serum |
| Kit Contents | Buffers, Capture antibody coated beads, Complete protocol, Diluents, Filter plate, Lot specific technical data sheet, Premixed detector antibody, SAV concentrate, Standard, Wash solution |
For Research Use Only. Not for use in diagnostic procedures.
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