The Mouse Amyloid Beta 42 (Ms Abeta 42) ELISA quantitates Ms Abeta 42 in human cerebrospinal fluid, tissue and cell culture supernatant. The assay will exclusively recognize both natural and recombinant Ms Abeta 42. Cross-reactivity has been observed in rat samples Principle of the method The Mouse Abeta 42 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.Beta-amyloid is a 40–43 amino acid peptide cleaved from amyloid precursor protein, and a putative gamma-secretase. Amyloid beta (1-40) together with Amyloid beta (1-42) are two major C-terminal variants of the amyloid beta protein, constituting the majority of amyloid beta proteins. Both the amyloid beta (40) and amyloid beta (42) alloforms are major components of the extracellular plaques found in Alzheimer's disease brain tissue.
Shipping condition: Wet ice
|Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers|
|2°C to 8°C|
|3.12 to 200pg/mL|
|Cerebrospinal Fluid,10 μL|Supernatant, 10 μL|Tissue Homogenate,10 μL|
|1 hr. 20 min.|
|Colorimetric Microplate Reader|
For Research Use Only. Not for use in diagnostic procedures.