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Invitrogen™ IL-1 beta Human ELISA Kit

Quantifies natural and recombinant human IL-1β in serum, buffered solution, or cell culture medium

£477.00 - £1412.00

Specifications

Accession Number P01584
Assay Range 3.9-250 pg/mL
Assay Sensitivity 1 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Products 3
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Product Code Brand Quantity Price Quantity & Availability  
10130573
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Invitrogen™
KHC0011
96 Tests
£477.00
96 tests
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10150573
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Invitrogen™
KHC0012
2 x 96 Tests
£618.00
Each
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10140573
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Invitrogen™
KHC0011C
5 x 96 Tests
£1412.00
Pack of 5
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Description

Description

The Human Interleukin-1beta (Hu IL-1beta) ELISA quantitates Hu IL-1β in human serum, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-1β. Principle of the method The Human IL-1β solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-1β is important in immune response.

Specifications

Specifications

P01584
1 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
IL-1,IL1-BETA,IL1F2
4.4%
HRP
RUO
2°C to 8°C
1 hr. 20 min.
3.9-250 pg/mL
Biotin
Serum, Supernatant
Colorimetric
3553
6.6%
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
1pg/mL
Serum, 50 μL; Supernatant, 50 μL
Human
2 hr. 55 min.
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