missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ IL-1 beta Human ELISA Kit

Quantifies natural and recombinant human IL-1β in serum, buffered solution, or cell culture medium

Brand:  Invitrogen™ KHC0011

Code : 50

Additional Details : Weight : 0.47000kg

 View more versions of this product

Product Code. 10130573

  • £400.00 / 96 tests


Estimated Shipment: 02-11-2021
Add to basket

For Research Use Only. All usage must comply with product instructions.
 

Description

Description

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
Specifications

Specifications

P01584
ELISA Kit
3.9-250 pg/mL
3553
1pg/mL
Serum, 50 μL; Supernatant, 50 μL
1 hr. 20 min.
Human
4.4%
Human
1 pg/mL
Colorimetric Microplate Reader
HRP
Biotin
Colorimetric
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
RUO
2°C to 8°C
2 hr. 55 min.
6.6%
96 Tests
Supernatant
IL-1,IL1-BETA,IL1F2
Videos
SDS
Documents
Certificates