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Invitrogen™ IL-12 p70 Rat ELISA Kit

A lymphokine

Brand:  Invitrogen™ KRC0122

Code : 50

Additional Details : Weight : 0.50000kg

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Product Code. 10069362

  • £497.00 / Each


Estimated Shipment
07-10-2020
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For Research Use Only. All usage must comply with product instructions.
 

Description

Description

Interleukin-12 (IL-12) is a heterodimeric 70 kDa cytokine composed of two covalently linked, glycosylated chains with molecular weights of 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. Studies have established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. IL-12 p70 is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Human and mouse IL-12 p40 share about 70% amino acid sequence homology.
Specifications

Specifications

Q9R103
Biotin
64546, 84405
ELISA Kit
RUO
100 μL
2°C to 8°C
4 hr.
Il12a, Il12b
4.2%
Rat
HRP
15.6-1000 pg/mL
Colorimetric
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
<3pg/mL
<3 pg/mL
Plasma, 10 μL|Serum, 10 μL|Supernatant, 100 μL
1 hr. 20 min.
Rat
5.1%
2 x 96 Tests
Supernatant
Colorimetric Microplate Reader
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For Research Use Only. Not for use in diagnostic procedures.