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Invitrogen™ EGF Human ELISA Kit

Quantifies natural or recombinant human Epidermal Growth Factor (EGF) in serum, plasma, buffered solution, cell culture medium or urine

£477.00 - £610.00

Specifications

Accession Number P01133
Assay Range 3.9-250 pg/mL
Assay Sensitivity <1 pg/mL
Conjugate Biotin
Product Type ELISA Kit
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Includes: Pre-coated plate, standard, detector antibody, Streptavidin-HRP, diluents, wash buffer, chromogen, stop solution, and plate covers.

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Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
10405143
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Invitrogen™
KHG0061
96 Tests
£477.00
96 tests
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Estimated Shipment: 23-04-2024
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10039172
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Invitrogen™
KHG0062
2 x 96 Tests
£610.00
192 tests
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Estimated Shipment: 12-04-2024
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Description

Description

The Human Epidermal Growth Factor (EGF) ELISA quantitates Hu EGF in human serum, plasma, buffered solution, urine, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu EGF. Principle of the method The Human EGF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

EGF is a member of a family of EGF-related growth factors.

Cell Analysis, Immunoassays, Ready-To-Use Immunoassay

Order Info

Shipping Condition: Room Temperature

Specifications

Specifications

P01133
<1 pg/mL
ELISA Kit
Human
Colorimetric Microplate Reader
HOMG4,URG
5.3%
Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Biotinylated Detection Antibody, Streptavidin-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
EGF
Plasma, 100 μL; Serum, 100 μL; Supernatant, 100 μL; Urine,10 μL
Human
4 hr.
3.9-250 pg/mL
Biotin
Plasma, Serum, Supernatant, Urine
Colorimetric Detection
1950
4 hr.
4.4%
HRP
RUO
2°C to 8°C
1 hr. 20 min.
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.