Invitrogen™ AcTEV™ Protease

Specifically recognizes a seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly, cleaving between Gln and Gly), ideal for removing affinity tags from fusion proteins

Brand:  Invitrogen™ 12575015

Product Code. 10216572

Description

An improved version of Tobacco Etch Virus (TEV) AcTEV™ Protease is highly site-specific, highly active and significantly more stable than native TEV protease, resulting in enhanced long-term activity.

• Highly specific cleavage activity
• Enhanced enzyme stability for prolonged protease activity
• Activity over a broad temperature (4°C to 30°C) and pH (6.0 to 8.5) range
• Six-histidine sequence to facilitate its removal from the digested protein sample
• Greater than 85% single-band purity with no nonspecific protease contamination

• Application
• Incubation with AcTEV™ Protease releases the protein of interest from the fusion tag
• This is an effective way to remove solubility, secretion, detection, and purification tags from recombinant protein
Enzyme specifications
• Purified from E. coli expressing the AcTEV™ Protease gene
• Unit definition
• One unit of AcTEV™ Protease cleaves 85% of a 3μg control substrate in 1 hr at 30°C
• Unit reaction conditions
• 50mM Tris-HCl (pH 8.0), 0.5mM EDTA, 1mM DTT, 3μg control substrate, and 1 unit enzyme in 30μL for 1 hr at 30°C AcTEV™ Protease is functionally tested for the absence of any nonspecific protease activity

GST-Tagged Protein Purification, His-Tagged Protein Purification, Protein and Peptide Purification, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis

Specifications

• Concentration: 1,000 Units
• Shipping Condition: Dry Ice
• Compatible Buffer: TEV Buffer
• Quantity: 1000 U
• Product Line: AcTEV™

• Content And Storage: AcTEV™ Protease is supplied with a vial of 20X TEV buffer [1 M Tris-HCl (pH 8.0), 10 mM EDTA] and a vial of 100 mM DTT.
  
  Store at -20° C. Guaranteed stable for 1 year when properly stored.
• Enzyme: TEV Protease
• Optimal Reaction Temperature: +4°C to +30°C
• Product Type: Protease

For Research Use Only. Not for use in diagnostic procedures.