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Novex™ Novex™ Zymogram Plus (Gelatin) Protein Gels, 10%, 1.0 mm
Description
Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases. Novex 10% Zymogram Plus gels are highly sensitive, detecting as little as 5 x 10-6 units of collagenase.
Formulation
Novex Zymogram Plus gels are Tris-glycine gels with 0.1% gelatin incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water, and gelatin as the substrate. All have a 4% stacking gel.
Choose the right gel format for your experiments
Novex 10% Zymogram Plus gels come in a fixed concentration of 10% gelatin and three different well formats, including 10-, 12- and 15-well.
Recommended buffers
Use Novex Tris-Glycine SDS Sample Buffer (LC2676) and Novex Tris-Glycine SDS Running Buffer (LC2675) with these gels. Novex Zymogram Renaturing Buffer (LC2670) and Novex Zymogram Developing Buffer (LC2671) are also recommended.
Recommended Storage
Store in refrigerator (2-8°C). Do not freeze.
Specifications
Specifications
| For Use With (Equipment) | Mini Gel Tank, XCell SureLock™ Mini-Cell |
| Gel Size | Mini |
| Product Line | Novex |
| Gel Percentage | 0.1 |
| Gel Type | Zymogram Gel |
| Mode of Separation | Molecular Weight |
| No. of Wells | 10-well |
| Quantity | 10 gels (1 box) |
| Sample Loading Volume | Up to 25 μL |
| Separation Range | 30 to 150 kDa (Tris-Glycine Buffer) |
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