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Thermo Scientific North2South™ Chemiluminescent Hybridization and Detection Kit

Acquire consistent, highly sensitive Southern blot results with a novel ECL HRP substrate and optimized hybridization and blocking buffers.

Brand:  Thermo Scientific 37555

UNSPSC : 41105326

Code : PN

Additional Details : CAS Number : 1193-72-2 Weight : 0.80000kg

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Product Code. 11869320

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Description

Description

The Thermo Scientific North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin-HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.

Highlights:

  • Sensitivity equal to or greater than 32P
  • Amenable to many formats
  • High level of light output and consistent low background allows for very short exposure times using cooled CCD camera systems or film
  • Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
Specifications

Specifications

Non-radioactive Southern and Northern blotting
Yields 60mM sodium citrate, 600mM sodium chloride, 0.2% SDS, pH 7.9 when diluted 1:1 in water
North2South Hybridization Stringency Wash Buffer (2X)
375mL
Documentation

Technical Documents