Thermo Scientific™ North2South™ Chemiluminescent Hybridization and Detection Kit

Acquire consistent, highly sensitive Southern blot results with a novel ECL HRP substrate and optimized hybridization and blocking buffers.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 17097

UNSPSC: 41105326

Code: PN

Additional Details:
Additional Details: Weight: 2.72160kg



Product Code. 10239423

Quantity Price
1 £ 350.0 / 1 set
EU Stock 3
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  • Description and Specification
  • Documentation
  • Accessories and Spare Parts
Description and Specification

Specification

Includes Stabilized Streptavidin-Horseradish Peroxidase Conjugated, 1.5mL
Luminol/Enhancer Solution, 80mL
Stable Peroxide Solution, 80mL
Hybridization Buffer, 125mL
(2X) Hybridization Stringency Wash Buffer, 375mL
Blocking Buffer, 500mL
(4X) Wash Buffer, 500mL
Substrate Equilibration Buffer, 500mL
Formulation Multi-component kit
Product Type North2South Chemiluminescent Hybridization and Detection Kit
For Use With (Application) Non-radioactive Southern and Northern blotting
Quantity 2160mL kit
No. of Reactions 10 mini-gel blots

The Thermo Scientific North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin-HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.

Highlights:

  • Sensitivity equal to or greater than 32P
  • Amenable to many formats
  • High level of light output and consistent low background allows for very short exposure times using cooled CCD camera systems or film
  • Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user

Documentation

Technical documents

Accessories and Spare Parts