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Thermo Scientific™ North2South™ Hybridization Buffer

Acquire consistent, highly sensitive Southern blot results with a novel ECL HRP substrate and optimized hybridization and blocking buffers.

Brand:  Thermo Scientific™ 37549

Code : PN

Additional Details : CAS Number : 3326-32-7 Weight : 2.72160kg

Product Code. 10066213

  • £120.00 / 125mL

In stock, ready to ship: 5

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Description

Description

The Thermo Scientific North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin-HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.

Highlights:

  • Sensitivity equal to or greater than 32P
  • Amenable to many formats
  • High level of light output and consistent low background allows for very short exposure times using cooled CCD camera systems or film
  • Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
Specifications

Specifications

Hybridization Buffer
125mL
Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.
North2South™
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