Thermo Scientific North2South™ Chemiluminescent Hybridization and Detection Kit

Acquire consistent, highly sensitive Southern blot results with a novel ECL HRP substrate and optimized hybridization and blocking buffers.

Overview
Brand: Thermo Scientific

Manufacturer Part Number: 37549

125 ML HYBRIDISATION BUFFER, NORTH2SOUTH 125ML

UNSPSC: 41105326

Code: PN

Additional Details:
Additional Details: CAS Number: 3326-32-7 Weight: 2.72160kg



Product Code. 10066213

Quantity Price
1 £ 98.5 / 125mL
EU Stock 5
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Description and Specification

Specification

For Use With (Application) Non-radioactive Southern and Northern blotting
Formulation Proprietary buffered solution, pH 6.9
Product Type North2South Hybridization Buffer
Quantity 125mL

The Thermo Scientific North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin-HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.

Highlights:

  • Sensitivity equal to or greater than 32P
  • Amenable to many formats
  • High level of light output and consistent low background allows for very short exposure times using cooled CCD camera systems or film
  • Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user

Documentation

Technical documents