Invitrogen™ Nop25 Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA589730

Product Code. 16567511

Description

Immunogen sequence: MGRNKKKKRD GDDRRPRLVL SFDEEKRREY LTGFHKRKVE RKKAAIEEIK QRLKEEQRKL REERHQEYLK MLAEREEALE EADELDRLVT AKTESVQYDH PNHTVTVTTI SDLDLSGARL LGLTPPEGGA GDRSEEEASS TEKPTKALPR KSRDPLLSQR ISSLTASLHA HSRKKVKRKH PRRAQDSKKP PRAPRTSKAQ RRRLTGKARH SGE; Positive Samples: Raji, A-431, HeLa, HepG2, SH-SY5Y; Cellular Location: Nucleus, nucleolus.

Nop25 is a nucleolar protein predominantly involved in the architecture and function of the nucleolus, which is the cellular hub for ribosomal RNA (rRNA) synthesis and ribosome assembly. It is identified as a putative RNA-binding protein and plays a crucial role in rRNA transcription. Nop25 has been characterized through molecular cloning, showing its ability to bind single-stranded nucleic acids directly. Experimental studies, such as those involving mouse embryogenesis, have utilized techniques like RT-PCR to analyze Nop25's gene expression across developmental stages. Moreover, research on the protein's structure has unveiled its nuclear and nucleolar targeting sequences, vital for its localization to the nucleolus. Treatment of cells with RNase A has illustrated the dissociation of Nop25 from the nucleolus, further highlighting its RNA-binding capabilities. This protein's functional importance is underscored by its role in maintaining nucleolar architecture, as demonstrated by experiments where overexpression of Nop25's N-terminal amino acids disrupted nucleolar integrity.

Specifications

• Antigen: Nop25
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NOL12
• Gene Alias: 25 kDa nucleolar protein; C78541; dJ37E16.7; Nol12; Nop25; Nucleolar protein 12; nucleolar protein of 25 kDa; nucleolar protein, 25 kDa; RGD1305327
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 79159
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 1.22 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: Q9UGY1
• Gene Symbols: NOL12
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-213 of human NOL12 (NP_0772891).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG