Invitrogen™ Human NLK, GST Tag Recombinant Protein

Product Code. 11523647

Brand: Invitrogen™ PV4309

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Recombinant, Full Length

NLK is a serine/threonine kinase involved in signal transduction and cellular communication. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors.

Specifications

• Accession Number: Q9UBE8
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50 mM tris HCl with 0.02% Triton X-100, 0.5 mM EDTA, 150 mM NaCl, 2 mM DTT, 50% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 51701
• Molecular Weight (g/mol): 84.5 kDa
• Name: Human NLK, GST Tag
• Quantity: 10 μg
• Regulatory Status: For research use only. Not for use in diagnostic procedures
• Gene Alias: AI194375; LAK1; nemo like kinase; nemo-like kinase; NLK; Protein LAK1; RGD1561602; serine/threonine protein kinase NLK; serine/threonine-protein kinase NLK
• Common Name: NLK
• KinaseFamily: MAP
• Product Type: NLK Recombinant Human Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Expression System: Insect cells
• Protein Family: Kinase and inhibitors
• Form: Liquid
• Protein Subtype: Serine/threonine kinase