NF-H Antibody (RT97), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 1 publication

Brand:  Novus Biologicals NBP2-29435-20ug

Product Code. 18464302

Description

NF-H Monoclonal specifically detects NF-H in Human, Mouse, Rat, Porcine, Chicken samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: NF-H
• Classification: Monoclonal
• Concentration: 0.2 mg/ml
• Dilution: Western Blot 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells, ELISA 1-5 ug/mL for coating, Immunohistochemistry, Immunohistochemistry-Paraffin 0.5-1.0 ug/mL, Immunohistochemistry-Frozen 0.5-1.0 ug/mL
• Gene Alias: 200 kDa neurofilament protein, KIAA0845, Neurofilament Heavy (200kDa), neurofilament heavy polypeptide, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa, NF200, NFH, NF-H
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This monoclonal antibody reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: RT97
• Conjugate: Unconjugated
• Gene Accession No.: P12036
• Gene Symbols: NEFH
• Immunogen: Triton-X 100 insoluble protein fraction of rat brain (Uniprot: P12036)
• Quantity: 20 ug
• Research Discipline: Autophagy, Cellular Markers, Cytoskeleton Markers, Neurodegeneration, Neurofilaments, Neuronal Cell Markers, Neuroscience
• Gene ID (Entrez): 4744
• Target Species: Human, Mouse, Rat, Pig, Chicken
• Form: Purified

For Research Use Only