Nestin, clone: 10C2, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9843-80
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal antibody 10C2 recognizes human nestin (residues 1464-1614). Nestin is a 220-240kDa Class VI intermediate filament protein that is expressed in stem cells of the developing nervous system. Expression has also been found in pancreatic islet and mesenchymal precursors in addition to cells with angiogenic potential. Upon differentiation, nestin-containing filaments are replaced by cell type-specific intermediate filament proteins, such as GFAP. Expression has been identified in a variety of cancerous cells including glioblastomas and pancreatic tumors. No cross-reactivity to rodent nestins has been observed using the 10C2 antibody.Applications Reported: This 10C2 antibody has been reported for use in immunocytochemistry, microscopy, western blotting, immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and flow cytometric analysis.Applications Tested: This 10C2 antibody has been tested by western blot on SDS-reduced and non-reduced lysates from U251 cells and can be used at less than or equal to 5 ug/mL. The 10C2 antibody has also been tested by immunocytochemistry on formaldehyde-fixed and permeabilized SKNSH cells and can be used at less than or equal to 5 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|ImmunocytochemistryþImmunohistochemistryþImmunohistochemistry (Frozen)þMicroscopyþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.