NEFM Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA110015

Product Code. 11565862

Description

In Western blot, this antibody detects bands at ∽145 kDa in crude homogenates of rat cerebellum homogenate. Human and bovine run slower at ∽160 kDa. A minor band at1∽110 kDa is also frequently seen, which appears to be an in-vivo proteolytic fragment of NF-M.

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Specifications

• Antigen: NEFM
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: NEFM
• Gene Alias: 160 kDa neurofilament protein; major neuronal intermediate filament subunit; medium neurofilament subunit; middle molecular weight neurofilament protein NF-M(2); Nef3; Nefm; nef-m; nefm.S; nefm-a; nefm-b; Neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; neurofilament M subunit; neurofilament medium; neurofilament medium polypeptide; neurofilament medium S homeolog; neurofilament medium tail domain; neurofilament protein M; Neurofilament protein, middle polypeptide; neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide S homeolog; neurofilament-3 (150 kD medium); neurofilament-M; neurofilament-M subunit; neuronal intermediate filament; NF160; NF165; NFM; NF-M; NF-M c-terminus; NF-M protein; NMC; XELAEV_18020229mg
• Host Species: Rabbit
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Bovine, Chicken, Equine, Human, Mouse, Porcine, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Concentration: Conc. Not Determined
• Formulation: whole serum with 5mM sodium azide
• Gene Accession No.: O77788, P07197, P08552, P08553, P12839, P16053
• Gene Symbols: NEFM
• Immunogen: Recombinant fusion protein containing the extreme C-terminal segment of rat NF-M, residues 549-845.
• Regulatory Status: RUO
• Gene ID (Entrez): 100054129, 100625407, 18040, 24588, 281347, 396206, 4741
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid