Invitrogen™ NDFIP2 Monoclonal Antibody (10D6D7)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA549299

Product Code. 17921811

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human A549 whole cell, human HepG2 whole cell, human MCF-7 whole cell, human Hela whole cell. IHC: human placenta tissue, human squamous cell lung carcinoma tissue, human breast cancer tissue, human spleen tissue. ICC/IF: A431 cell. Flow: JK cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus and multivesicular body where it mediates KCNH2 degradation (PubMed:26363003). May modulate EGFR signaling. Together with NDFIP1, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination. [UniProt].

Specifications

• Antigen: NDFIP2
• Classification: Monoclonal
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: Q9NV92
• Gene Symbols: NDFIP2
• Immunogen: E.coli-derived human NDFIP2 recombinant protein (Position: M16-L336).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2b

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Clone: 10D6D7
• Conjugate: Unconjugated
• Gene: NDFIP2
• Gene Alias: 0710001O20Rik; 2810436B12Rik; 9130207N19Rik; KIAA1165; MAPK-activating protein PM04 PM05 PM06 PM07; mKIAA1165; N4wbp5a; NDFIP2; Nedd4 family interacting protein 2; NEDD4 family-interacting protein 2; NEDD4 WW domain-binding protein 5A; NF-kappa-B-activating protein 413; putative MAPK-activating protein PM04/PM05/PM06/PM07; Putative NF-kappa-B-activating protein 413; Unknown (protein for IMAGE:7958216); wu:fa92h03; wu:fk31f07; zgc:63948
• Host Species: Mouse
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 54602
• Content And Storage: -20°C
• Form: Lyophilized