The NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I).

Brand: Thermo Scientific

Manufacturer Part Number: ER0571

NCOI 10U/UL 500U

UNSPSC: 12352204

Code: 88

Additional Details:
Additional Details: Weight: 0.00900kg

Product Code. 10233160

Quantity Price
1 £24.97 / 500 units
EU Stock 71
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Description and Specification


Product Name NcoI
Concentration 10U/µL
Units 500U

5'...C▵C A T G G...3'

3'...G G T A C▵C...5'

Conditions for 100% Activity:

  • 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • NcoI is supplied in: 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with NcoI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Compatible Ends:

  • AflIII, PscI, BtgI, Eco130I, FatI, PagI


Low salt, high glycerol (>5%) concentrations, pH>8.0 or a large excess of enzyme may result in star activity.