Nanog Rat anti-Mouse, eFluor 660, Clone: eBioMLC-51, eBioscience™

Rat Monoclonal Antibody

Brand: Affymetrix eBioscience

Manufacturer Part Number: 50-5761-80

25UG Anti-Mouse Nanog eFluor 660

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg

Disclaimers: For Research Use Only.

Product Code. 15529986

Quantity Price
1 £138.80 / 25µg
Estimated Shipment date
from Supplier 27-10-2016
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Description and Specification


Antigen Nanog
Applications Flow Cytometry (Intracellular Staining)
Applications Immunocytochemistry
Applications Immunohistochemistry
Applications Microscopy
Applications Western Blotting
Clone eBioMLC-51
Concentration 0.2mg/mL
Conjugate eFluor 660
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Host Species Rat
Isotype IgG2a
Quantity 25μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The eBioMLC51 monoclonal antibody recognizes mouse Nanog. Nanog is a multidomain homeobox transcription factor that has been shown to maintain pluripotency of embryonic stem cells, independent of LIF/Stat3. Expression of Nanog in the mouse is specific to early embryos, the ICM of the blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells. Nanog expression often overlaps, but is not identical to, that of Oct4. Nanog is downregulated upon cellular differentiation and loss of pluripotency, making it a suitable marker in determining the undifferentiated state of stem cells.

Nanog acts as a transcriptional activator and has two activation domains in the C-terminus, called CD2 and WR, and one activation domain in the N terminus. The CD2 domain is unique to Nanog, whereas the NK2 DNA binding domain is well conserved. Immunoblotting using eBioMLC51 reveals a band at ∽ 45kDa in F9 (an embryonal carcinoma cell line) lysate, but not in lysate from the NIH3T3 cell line or mouse spleen. Preliminary data using fluorochrome-conjugated eBioMLC51 suggests that it is essential to use the eBioscience Foxp3 Staining Buffer Set, Cat. 00-5523, for intracellular staining of mouse Nanog.