Nanog Monoclonal Antibody (eBioMLC-51), eFluor™ 660, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-5761-82

Product Code. 15539986

Description

Description: The eBioMLC51 monoclonal antibody recognizes mouse og. og is a multidomain homeobox transcription factor that has been shown to maintain pluripotency of embryonic stem cells, independent of LIF/Stat3. Expression of og in the mouse is specific to early embryos, the ICM of the blastocyst, embryonic stem (ES) cells, and embryonic germ (EG) cells. og expression often overlaps, but is not identical to, that of Oct4. og is downregulated upon cellular differentiation and loss of pluripotency, making it a suitable marker in determining the undifferentiated state of stem cells. og acts as a transcriptional activator and has two activation domains in the C-terminus, called CD2 and WR, and one activation domain in the N terminus. The CD2 domain is unique to og, whereas the NK2 DNA binding domain is well conserved. Immunoblotting using eBioMLC51 reveals a band at ∽ 45 kDa in F9 (an embryonal carcinoma cell line) lysate, but not in lysate from the NIH3T3 cell line or mouse spleen. Preliminary data using fluorochrome-conjugated eBioMLC51 suggests that it is essential to use the eBioscience Foxp3 Staining Buffer Set, Cat. 00-5523, for intracellular staining of mouse og. Applications Reported: This eBioMLC-51 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, western blotting, microscopy, immunohistochemical staining, and immunocytochemistry.

Nanog Monoclonal antibody specifically detects Nanog in Mouse samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot.

Specifications

• Antigen: Nanog
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q80Z64
• Gene Symbols: NANOG
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 71950
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot
• Clone: eBioMLC-51
• Conjugate: eFluor 660
• Gene: NANOG
• Gene Alias: 2410002E02Rik; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein; Ecat4; ENK; ES cell-associated protein 4; FLJ12581; HGNC:20857; hNanog; Homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog; Nanog homeobox
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

For Research Use Only.