PeproTech NAMPT Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Product Code	Brand	Quantity
17875083	PeproTech 500-P222BT-25UG	25 μg
17895783	PeproTech 500-P222BT-50UG	50 μg
17864923	Invitrogen 500-P222BT-1MG	2 x 500 μg
30054396	PeproTech 500-P222BT-500UG	500 μg

Description

AA Sequence of recombinant protein: MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Visfatin. Anti-Human Visfatin-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human Visfatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Visfatin (500-P222) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Visfatin. Western Blot: To detect Human Visfatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Visfatin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P222BT-1MG will be provided as 2 x 500 μg

Visfatin, also known as pre-B cell colony-enhancing factor, is an adipocytokine that is abundantly secreted by visceral adipose tissue, with insulin mimetic effects. Visfatin mimics insulin signaling by binding to the insulin receptor and reducing glucose release from liver cells, while stimulating glucose utilization in adipocytes and myocytes. Visfatin is also invoved in enhancement of cell proliferation, biosynthesis of nicotinamide mono-and dinucleotide and glucose homeostasis.

Specifications

• Antigen: NAMPT
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: P43490
• Gene ID (Entrez): 10135
• Immunogen: E.coli-derived, 52.6 kDa Recombinant Human Visfatin.
• Purification Method: Antigen affinity chromatography
• Gene: NAMPT

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: 1110035O14Rik; AI314458; AI480535; DKFZp666B131; EC 2.4.2.12; hypothetical protein; MGC117256; NAmPRTase; NAMPT; nampt1; nampta; NAMPTP1; Nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase 1; nicotinamide phosphoribosyltransferase a; nicotinamide phosphoribosyltransferase pseudogene 1; PBEF; Pbef1; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1; Pre-B-cell colony-enhancing factor 1 homolog; pre-B-cell colony-enhancing factor 1 precursor; VF; VISFATIN
• Gene Symbols: NAMPT
• Primary or Secondary: Primary
• Content And Storage: -20°C