Invitrogen™ N-terminal Arginylation Monoclonal Antibody (4A9)

Mouse Monoclonal Antibody

Brand:  Invitrogen™ MA527563

Product Code. 16302084

Description

MA5-27563 is specific for N-terminal arginine next to glutamic acid. It does not detect N-terminal arginine next to aspartic acid or internal arginine residues. A 1:1,000 dilution of MA5-27563 was sufficient for detection of N-terminal Arginylation in 0.5 μg of N-terminal Arginine peptide conjugated to BSA by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.

Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood. Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress.

Specifications

• Antigen: N-terminal Arginylation
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.09% sodium azide; pH 7.4
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Target Species: Chemical
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Western Blot
• Clone: 4A9
• Conjugate: Unconjugated
• Gene Alias: N terminal Arginine; N-terminal Arginine
• Immunogen: Synthetic N-terminal arginylated peptide conjugated to KLH.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: -20°C
• Form: Liquid
• Target Molecule: Chemical