Invitrogen™ MYO1A Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA529084

Product Code. 13433207

Description

Recommended positive controls: Jurkat, Raji, K562, NCI-H929. Predicted reactivity: Mouse (93%), Rat (94%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The protein encoded by this gene belongs to the myosin superfamily. Myosins are molecular motors that, upon interaction with actin filaments, utilize energy from ATP hydrolysis to generate mechanical force. Each myosin has a conserved N-terminal motor domain that contains both ATP-binding and actin-binding sequences. Following the motor domain is a light-chain-binding 'neck' region containing 1-6 copies of a repeat element, the IQ motif, that serves as a binding site for calmodulin or other members of the EF-hand superfamily of calcium-binding proteins. At the C-terminus, each myosin class has a distinct tail domain that serves in dimerization, membrane binding, protein binding, and/or enzymatic activities and targets each myosin to its particular subcellular location. The kidney epithelial cell line, LLC-PK1-CL4 (CL4), forms a well ordered brush border (BB) on its apical surface. Experiments indicate that the brush border population of the encoded protein turns over rapidly, while its head and tail domains interact transiently with the core actin and plasma membrane, respectively. A rapidly exchanging pool of the protein encoded by this gene envelops an actin core bundle that, by comparison, is static in structure.

Specifications

• Antigen: MYO1A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MYO1A
• Gene Alias: BBMI; BBM-I; brush border myosin 1; brush border myosin I; brush border myosin-I; DFNA48; MIHC; Myh1; MYHL; Myo1a; myosin heavy polypeptide 1 skeletal muscle adult; myosin I heavy chain; myosin IA; myosin, heavy polypeptide-like (100kD); myosin, heavy polypeptide-like (110kD); myosin-Ia; unconventional myosin-Ia
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 4640
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.68 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9UBC5
• Gene Symbols: MYO1A
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Myosin 1A. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG