Myc Tag Monoclonal Antibody, AP, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen R95225

Product Code. 10236152

Description

NOTE: R952-25 was previously sold under product #46-0285. When re-ordering, please use #R952-25. This antibody recognizes amino acid sequence: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-. This product contains enough material for 25 Western blots. Western blot experiments with 100 ng of recombinant Positope™ control protein gave a detectable signal within 15 minutes using a chromogenic substrate. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5. For Western blot, we recommend diluting the AP-conjugated antibody in Tris-Buffered Saline (TBS) containing 0.1% (v/v) Tween-20 and 1% (w/v) nonfat dry milk. In Western blot applications, no cross-reactivity has been observed in bacterial lysates.

Myc is a proto-oncogene and encodes a nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. The encoded protein forms a heterodimer with the related transcription factor MAX. This complex binds to the E box DNA consensus sequence and regulates the transcription of specific target genes. Amplification of this gene is frequently observed in numerous human cancers. Translocations involving this gene are associated with Burkitt lymphoma and multiple myeloma in human patients. There is evidence to show that translation initiates both from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site, resulting in the production of two isoforms with distinct N-termini.

Specifications

• Antigen: Myc Tag
• Classification: Monoclonal
• Conjugate: Alkaline Phosphatase (AP)
• Gene: MYC
• Gene Alias: 46-0603; AU016757; avian myelocytomatosis viral oncogene homolog; bHLHe39; c Myc; class E basic helix-loop-helix protein 39; cmyc; c-Myc; c-myc epitope tag; c-myc proto-oncogene; MRTL; MYC; Myc protein; Myc proto-oncogene protein; Myc Tag; Myc2; MYCC; myc-related translation/localization regulatory factor; myelocytomatosis oncogene; Niard; Nird; Proto-oncogene c-Myc; R95025; R950-25; transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog
• Host Species: Mouse
• Purification Method: purified
• Regulatory Status: RUO
• Gene ID (Entrez): 4609
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid
• Target Molecule: Tag

• Applications: Western Blot
• Concentration: 1-2 mg/mL
• Formulation: PBS with 0.01% sodium azide
• Gene Accession No.: P01106
• Gene Symbols: MYC
• Immunogen: c-Myc synthetic peptide: Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu-
• Quantity: 125 μL
• Primary or Secondary: Primary
• Target Species: Tag
• Product Type: Antibody
• Isotype: IgG1